Further, the desired gene expression was measured by immunoblotting. Cytokines were measured by ELISA while Iba-1 and GFAP expression were determined by immunofluorescence. Depressive-like behaviors were confirmed via behavior tests including OFT, FST, SPT, and TST. We investigated the involvement of HDAC1 and eEF2 in the antidepressant mechanisms of fluoxetine using a lipopolysaccharide (LPS)-induced depression-like behavior model.MethodsFor in vivo analysis, mice were treated with LPS (2 mg/kg BW), fluoxetine (20 mg/kg BW), HDAC1 activator (Exifone: 54 mg/kg BW) and NH125 (1 mg/kg BW). For the Y columns, choose A single column of values.BackgroundSelective serotonin reuptaker inhibitors, including fluoxetine, are widely studied and prescribed antidepressants, while their exact molecular and cellular mechanism are yet to be defined. For the X column, select None (column graph). In the lower half of the Welcome dialog, tell Prism how to format your data table. Uncheck the option box at the bottom of the Welcome dialog to Pop up new user hints. Choose to Create a new project and to Work independently. When you launch Prism, the Welcome to Prism dialog appears. If you have more than two groups, but the data are grouped by two factors, refer to step-by-step on two-way ANOVA. If you have only two data groups to compare, try the step-by-step example on t tests. To learn more about analysis of variance, consult the ANOVA sections of Analyzing Data with GraphPad Prism (pdf). In this step-by-step example, we'll demonstrate a simple repeated-measures ANOVA followed by the Tukey post test, but we'll point out other options as we go. Following nonparametric analysis, Prism can perform Dunn's post test. You don't have to memorize these assumptions if you answer the questions above for your experiment, Prism will pick the correct test for you.įollowing ANOVA, Prism can perform the Bonferroni, Tukey, Newman-Keuls, or Dunnett's post test.

Graphpad prism anova post hoc